Yes, I was asked two questions regarding it.
1) Are there anything else beside the speeding tickets?
2) Do you have proof that you've already paid for the speeding ticket?
For the first one I answered No as I didn't have anything else (if you do you but forgot to list it earlier you can say that here) and for the second one I said I've obtained my driving record from DMV which is clean. IO was satisfied with the answer and didn't ask to see DMV records and moved on to other parts of the interview.
So to answer your question, it wasn't an issue in my case. It could also depend on the IO though.